A Spectrophotometric Method for Measuring Serum Trypsin Inhibitor Capacity.
نویسندگان
چکیده
The spectrophotometric method of Schwert and Takenaka (11) for the assay of trypsinesterolytic activity hasbeen modified for the measurementof the trypsin inhibitor capacity of serum. An averagevalue of 0.99 mg. of trypsin inhibited per milliliter of serum (3 S.D. = ±0.30) wasdetermined for 50 control subjects. Patient serum assayvaluesdeterminedwith this method havebeenfoundsimilar to thosedetermined with the modified Kunitz method using l131-labeledcaseinas the substratein the normal range for a variety of pathologicconditionsand in the elevated range for abnormalities of the pancreas and generalized cancer conditions. From the standpointsof accuracy, simplicity, and speed this technic is believed to be an improvementover previousmethodsfor the measurementof this serum variable.
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عنوان ژورنال:
- Clinical chemistry
دوره 12 شماره
صفحات -
تاریخ انتشار 1963